DNA

Part:BBa_K5300045:Design

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-10-01)


Composite part composed of PglnK and gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 714


Design Notes

To verify the ability of the endogenous glnK promoter from Sinorhizobium fredii CCBAU45436 to initiate downstream gene expression under low-nitrogen conditions, we constructed a promoter-reporter system in pBBR1MCS-2 plasmid, using GFP as a reporter to reflect the promoter’s induction activity based on gfp expression levels.


Source

Sinorhizobium fredii CCBAU45436, Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)

References