DNA

Part:BBa_K5300039:Design

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-30)


PnifH-dipeptide aldehyde, composite part composed of PnifH and dipeptide aldehyde biosynthetic gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 8611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2543
    Illegal BglII site found at 3707
    Illegal BglII site found at 4379
    Illegal BglII site found at 6047
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2965
    Illegal NgoMIV site found at 3327
    Illegal NgoMIV site found at 4498
    Illegal NgoMIV site found at 5275
    Illegal NgoMIV site found at 6058
    Illegal NgoMIV site found at 8262
    Illegal NgoMIV site found at 8295
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2016
    Illegal BsaI site found at 2481
    Illegal BsaI site found at 3573
    Illegal BsaI site found at 6726
    Illegal BsaI site found at 7755
    Illegal BsaI.rc site found at 4771
    Illegal BsaI.rc site found at 6394
    Illegal BsaI.rc site found at 6511
    Illegal SapI site found at 4035
    Illegal SapI.rc site found at 1959


Design Notes

To validate the function of the nifH promoter, we constructed a composite circuit combining the nifH promoter and the dipeptide aldehyde synthesis gene cluster.


Source

Sinorhizobium fredii CCBAU45436, Clostridium sp. CAG:567（Human metagenome）

References