Plasmid

Part:BBa_K5300034

Designed by: yu peng   Group: iGEM24_CAU-China   (2024-09-29)


pJQ200SK, a suicide vector, is commonly used for gene deletion of rhizobia.

PJQ200SK is a small, mobilizable suicide plasmid which is commonly used in rhizobium gene deletion experiments. The gentamicin resistance carried by pJQ200SK can be used to screen for mutant strains that generate single exchange. Gene deletion mutant strains are obtained through homologous double exchange through passage culture. At the same time, single exchange mutant strains are removed using the sucrose lethal gene sacB on the plasmid. Finally, a gene deletion mutant with pJQ200SK removed is obtained.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5400
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5400
    Illegal NheI site found at 344
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5400
    Illegal BglII site found at 4190
    Illegal BamHI site found at 5383
    Illegal XhoI site found at 5332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5400
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5400
    Illegal XbaI site found at 5415
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1390
    Illegal NgoMIV site found at 1516
    Illegal NgoMIV site found at 1526
    Illegal NgoMIV site found at 1923
    Illegal AgeI site found at 430
    Illegal AgeI site found at 754
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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