Coding

Part:BBa_K5300031

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-29)


bgc33，a protein coding sequences derived from human metagenome，synthesizes nonribosoma

The complete sequence of bgc33 is derived from the Human Microbiome Project. The complete bgc33 encodes nonribosomal peptide synthetase (NRPS), which catalyzes the synthesis of N-octanoyl-Met-Phe-H, with the N-terminus protected by an acyl group. Addition of the acyl group is carried out by a condensed structural domain at the N-terminal end of the NRPS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1940
    Illegal BglII site found at 3104
    Illegal BglII site found at 3776
    Illegal BglII site found at 5444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2362
    Illegal NgoMIV site found at 2724
    Illegal NgoMIV site found at 3895
    Illegal NgoMIV site found at 4672
    Illegal NgoMIV site found at 5455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1413
    Illegal BsaI site found at 1878
    Illegal BsaI site found at 2970
    Illegal BsaI site found at 6123
    Illegal BsaI site found at 7152
    Illegal BsaI.rc site found at 4168
    Illegal BsaI.rc site found at 5791
    Illegal BsaI.rc site found at 5908
    Illegal SapI site found at 3432
    Illegal SapI.rc site found at 1356


[edit]
Categories
Parameters
None