DNA

Part:BBa_K5300029

Designed by: yu peng   Group: iGEM24_CAU-China   (2024-09-29)


Up-Arm phaC2

Up-Arm phaC2 is a DNA sequence located upstream of the phaC2 gene. During the experiment of phaC2 gene deletion, homologous recombination occurred between the homologous sequence and the upstream sequence of phaC2 gene. After screening and iteration by CAU-China, Up-Arm phaC2 was ultimately selected as the optimal homologous sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 206
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 446


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Parameters
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