DNA

Part:BBa_K5300028

Designed by: yu peng   Group: iGEM24_CAU-China   (2024-09-29)


DOWN-feo-suicide

Downstream homology arm element for integrating the suicide circuit sequence into the S. fredii CCBAU45436 genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 325
    Illegal NotI site found at 682
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 415
    Illegal XhoI site found at 757
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 325
    Illegal NgoMIV site found at 396
    Illegal AgeI site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None