Coding

Part:BBa_K5300021

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-23)


Cas12k, related to CRISPRi system

A member of the Cas protein family, lacks cleavage activity and inhibits transcription through physical binding without cutting the target sequence. When intracellular sgRNA levels decrease, the bound Cas12k protein detaches from the target sequence, allowing normal transcription to resume.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None