Composite

Part:BBa_K5299201:Design

Designed by: Maria - Asimina Kakadiari   Group: iGEM24_Thessaly   (2024-09-23)


P3.1 - BBa_B0034 - BBa_I746916 - BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 256


Design Notes

Constructed according to Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.

Source

The promoter, BBa_K4583008 is already in the Registry. It originally comes from Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491. The RBS, BBa_B0034 already exists in the Registry. The sfGFP, BBa_I746916, already exists in the Registry. The terminator, BBa_B0015, already exists in the Registry.

References

  • [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491