Part:BBa_K5299121

THI20 protein (V. dahliae)

Biology

THI20 (phosphomethylpyrimidine kinase) (XM_009655087.1) has a role in V. dahliae's thiamine biosynthesis pathway.

Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-β-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1].

Figure 1: Thiamine biosynthetic pathway.

Use

This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].
We designed this part to test if our bacteria could produce dsRNA targeting this gene.

References

[1] Qin, T., Hao, W., Sun, R., Li, Y., Wang, Y., Wei, C., … Wang, Q. (2020). Verticillium dahliae VdTHI20, involved in pyrimidine biosynthesis, is required for DNA repair functions and pathogenicity. International Journal of Molecular Sciences, 21(4), 1378. doi:10.3390/ijms21041378 Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 64
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]