Part:BBa_K5299120

AAC protein (V. dahliae)

Biology

The AAC (NW_009276936.1 VDAG_07535) gene codes for an ADP/ATP carrier protein that helps generate energy for V. dahliae. It is essential for maintaining the ADP/ATP balance by mediating the exchange of ADP and ATP between the mitochondria and the cytoplasm. [1]

Figure 1: ADP/ ATP carrier transports ATP out of the mitochondria into the cytoplasm and brings ADP back in.

Use

This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [2].

We designed this part to test if our bacteria could produce dsRNA that targets this protein.

References

[1]Bertholet, A. M., Chouchani, E. T., Kazak, L., Angelin, A., Fedorenko, A., Long, J. Z., Vidoni, S., Garrity, R., Cho, J., Terada, N., Wallace, D. C., Spiegelman, B. M., & Kirichok, Y. (2019). H+ transport is an integral function of the mitochondrial ADP/ATP carrier. In Nature (Vol. 571, Issue 7766, pp. 515–520). Springer Science and Business Media LLC. https://doi.org/10.1038/s41586-019-1400-3
[2] Su, X., Rehman, L., Guo, H., Li, X., Zhang, R., & Cheng, H. (2017). AAC as a Potential Target Gene to Control Verticillium dahliae. Genes, 8(1), 25. doi:10.3390/genes8010025

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 98
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]