Part:BBa_K5299119

RGS1 protein V. dahliae

Biology

VdRGS1 (MG583845.1) codes for a negative regulator of G protein signaling in V. dahliae and plays a role in many development stages of the fungus including spore germination, hyphal development, spore production, and microsclerotia formation [1].
The RGS1 protein is part of the G signaling pathway. By assisting the Gα subunit hydrolyse GTP it turns off the G protein-coupled signaling pathways and stops downstream signals [2].

Figure 1: RGS1 proteins accelerate the GTPase activity of Gα subunits, promoting the rapid conversion of GTP to GDP.

Use

This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1]. We designed this part to test if our bacteria could produce dsRNA targeting this fungal protein.

References

[1] Xu, J., Wang, X., Li, Y., Zeng, J., Wang, G., Deng, C., & Guo, W. (2018). Host-induced gene silencing of a regulator of G protein signalling gene (VdRGS1) confers resistance to Verticillium wilt in cotton. Plant Biotechnology Journal, 16(9), 1629–1643. doi:10.1111/pbi.12900
[2] Tuteja N. (2009). Signaling through G protein coupled receptors. Plant signaling & behavior, 4(10), 942–947. https://doi.org/10.4161/psb.4.10.9530

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 98