Composite

Part:BBa_K5299019

Designed by: Maria Nefeli Stoupa   Group: iGEM24_Thessaly   (2024-09-24)


T7terhyb6 reverse - T7ter pro- RGS1- T7ter pro reverse- T7terhyb6

By placing the T7 promoter and the T7hyb6 terminator from the gene of interest back to back and parallel and antiparallel we can produce dsRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 87


[edit]
Categories
Parameters
None