Plasmid_Backbone

Part:BBa_K5299004

Designed by: Maria Nefeli Stoupa   Group: iGEM24_Thessaly   (2024-09-22)


pSEVA63g19[gA]

pSEVA63g19[gA] is a modular plasmid from the SEVA (Standard European Vector Architecture) collection, specifically designed for genetic engineering in Gram-negative bacteria. The "63" indicates that it carries a gentamycin resistance gene for selection, while "3g" refers to the pBBR1 origin of replication, which supports stable replication across a broad range of bacterial species. The "[gA]" tag specifies that the plasmid's cargo is A13B, associated with level 2 reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 323
    Illegal XbaI site found at 296
    Illegal XbaI site found at 699
    Illegal SpeI site found at 761
    Illegal PstI site found at 284
    Illegal PstI site found at 711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 323
    Illegal SpeI site found at 761
    Illegal PstI site found at 284
    Illegal PstI site found at 711
    Illegal NotI site found at 46
    Illegal NotI site found at 729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 323
    Illegal BglII site found at 1426
    Illegal BamHI site found at 302
    Illegal BamHI site found at 693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 323
    Illegal XbaI site found at 296
    Illegal XbaI site found at 699
    Illegal SpeI site found at 761
    Illegal PstI site found at 284
    Illegal PstI site found at 711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 323
    Illegal XbaI site found at 296
    Illegal XbaI site found at 699
    Illegal SpeI site found at 761
    Illegal PstI site found at 284
    Illegal PstI site found at 711
    Illegal NgoMIV site found at 1969
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 76
    Illegal BsaI.rc site found at 684


[edit]
Categories
Parameters
None