Part:BBa_K5291049

AdhA

Alcohol dehydrogenases (ADHs) are a class of key enzymes responsible for the oxidation of alkyl alcohols in the aerobic alkane metabolic pathway. AdhA is a kind of alcohol dehydrogenases.

Usage and Biology

AdhA is from the system AlkB2-Rd45-AdhA, which is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.


Fig.1 The map of the plasmid pAB1-AlkB2-Rd45-AdhA.


Fig.2 The PCR result of AlkB-Rd45-AdhA fragment. The band was identycal to the expected length of 3033bp.

And we have conducted SDS-page of pAB1-AlkB2-Rd45-AdhA, which is expected to express a large fusion protein AlkB2-Rd45-AdhA with a molecular weight of 94.59kDa. In the figure, it can be seen that there were an extra bands in the experimental group than in the control group at a position slightly less than 100kDa, suggesting that AlkB2-Rd45-AdhA protein was successfully expressed.

Fig.3 SDS-PAGE result of AlkB2-Rd45-AdhA.

Since we set AlkB2 Rd45 and AdhA as a whole, the overall functions need moving to the part BBa_K5291033.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 769
  
• 1000
  COMPATIBLE WITH RFC[1000]