RNA
RiboJ

Part:BBa_K5291041

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-09-28)


RiboJ

RiboJ is a kind of self-cleaving ribozyme which contains 72 nucleotide sequence consisting of a satellite RNA of Tobacco ringspot virus(sTRSV) derived ribozyme followed by a hairpin sequence. It could be used as an insulator, which isolates parts from unwanted interactions with their neighboring regions. In our project. We used this part to isolate the expression boxes of proteins from one another in our component parts so that our proteins in component parts can express stably.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None