Composite
PEBP-GFP

Part:BBa_K5291036:Design

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)


pAB1-pS-PEBP-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
    Illegal NotI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1033
    Illegal BamHI site found at 996
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
    Illegal NgoMIV site found at 1351
    Illegal NgoMIV site found at 1720
    Illegal AgeI site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PEBP is able to bind to PE microplastics through non-specific, non-covalent hydrogen bonding and non-polar interactions. GFP enables us to view the binding situation under the fluorescence microscopy.

Source

The PEBP is predicted by the modeling group of BNUZH-China.

References