Composite
PEBP-PEase

Part:BBa_K5291035:Design

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)


pAB1-pS-PEBP-PEase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 110
    Illegal NotI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 110
    Illegal NgoMIV site found at 2695
    Illegal NgoMIV site found at 3064
    Illegal AgeI site found at 1363
    Illegal AgeI site found at 2347
    Illegal AgeI site found at 2410
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PEBP is able to bind to PE microplastics through non-specific, non-covalent hydrogen bonding and non-polar interactions. And the PEase is to degrade the PE microplastics into monomers.

Source

The PEBP is predicted by the modeling group of BNUZH-China.
The signal peptide and autotranspoter sequences come from Pseudomonas aeruginosa genomic sequence.

References

[1]Kuyukina, M. S., et al. (2015). "Trehalolipid biosurfactants from nonpathogenic Rhodococcus actinobacteria with diverse immunomodulatory activities." New Biotechnology 32(6): 559-568.
[2]Bergman, M. T., et al. (2023). "In Silico Design and Analysis of Plastic-Binding Peptides." The Journal of Physical Chemistry B 127(39): 8370-8381.