Part:BBa_K5291031

pAB1-pS-PAO102

A system used to deal with CO₂ released from Pseudomonas aeruginosa during PE degradation.

Usage and Biology

We have known from papers related to PE degradation that during this process a huge amount of CO₂ will be released, which is hard to solute in the water and exists risks of exceeding the absorption limit of mangroves. Therefore, we introduce this system to address the problem of CO₂. The gene PAO102 is native in Pseudomonas aeruginosa and can speed up the conversion between CO₂ and bicarbonate. And the promoter pS is verified that it is able to come into effect in P. aeruginosa.


Fig.1 The schema of pAB1-pS-PAO102.

We apply the method of PAO102 high copies, so that CO₂ produced by engineered P. aeruginosa can be swiftly conversed to bicarbonate, easily transporting in the biofilm.

Fig.2 The AGE figure of PAO102 colony PCR and the map of the plasmid.

However, there is a great pity that we have not finished the verification of the function due to rush time.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 110
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 110
  Illegal NotI site found at 291
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 110
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 110
  
• 1000
  COMPATIBLE WITH RFC[1000]