Part:BBa_K5291030

pBBRMCS2-acaP

We use this composite part to improve the efficiency of bicarbonate-CO₂ conversion in Rhodopseudomonas palustris CGA009, speeding up the process of carbon fixation.

Usage and Biology

The gene acaP in Rhodopseudomonas palustris is able to encode a kind of α-CAs that can speed up the conversion between CO₂ and bicarbonate. By the method of acaP high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO₂ conversion in R. palustris increase and the engineered bacteria is able to take advantage of carbon to produce cellulose. We make use of the promoter pT₇ to realize the constitutive expression of acaP. And BiTerm, the terminator which can come into effect in our engineered bacteria, is used in the system.


Fig.1 The schema of pS-acaP-BiTerm.

And we also successfully construct the strain with the complete plasmid.


Fig.2 The AGE figure of pBBR1MCS2-pS-acaP and the map of the plasmid.

It is a great pity that we have not finished the verification of the function due to rush time.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 242
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 242
  Illegal NotI site found at 845
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 171
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 242
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 242
  
• 1000
  COMPATIBLE WITH RFC[1000]