Part:BBa_K5291023:Design

BiTerm bidirectional terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The use of BiTerm provides insulation at both ends of the expression cassette, thereby reducing the influence of the surrounding genetic context on gene expression and enhancing the stability and efficiency of gene expression. Consequently, in our design, BiTerm is employed to mitigate the mutual influence of multi-gene expression.

Source

The sequence of BiTerm is derived from the article Nature methods:Characterization of 582 natural and synthetic terminators and quantification of their design constraints.

References

Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jul;10(7):659-64. doi: 10.1038/nmeth.2515. Epub 2013 Jun 2. PMID: 23727987.