Coding
RCA

Part:BBa_K5291006

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-08)


acaP

A gene from Rhodopseudomonas palustris CGA009 encoding a kind of carbonic anhydrase.

Usage and Biology

The gene acaP in Rhodopseudomonas palustris is able to encode a kind of α-CAs that can speed up the conversion between CO2 and bicarbonate. By the method of acaP high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO2 conversion in R. palustris increase and the engineered bacteria is able to take advantage of carbon to produce cellulose.


Fig.1 The schema of acaP.


Fig.2 The map of pBBR1MCS2-acaP.

However, there is a great pity that we have not finished the verification of the function due to rush time.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None