Composite

Part:BBa_K5286007

Designed by: Mara Valverde Rascón   Group: iGEM24_TUM-Straubing   (2024-10-02)


CAHS94063+pT7max

This part expresses tardigrade protein under T7max promoter and it when coexpressed with the autolysate pAD-LyseR plasmid According to Dydovik et al., 2017 and Sun et al., 2013, the lysate produced can be desiccated and will last stored at a room temperature for weeks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/composite/uncategorized
Parameters
chassisE. coli BL21(DE3)