Coding

Part:BBa_K5280129:Design

Designed by: Hua XU   Group: iGEM24_HKUST-GZ   (2024-09-07)

LexRO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
    Illegal NotI site found at 587
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
    Illegal PstI site found at 445
    Illegal PstI site found at 514
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did reverse translate and make sure it is iGEM Type II compatible.

Source

The sequence was firstly published in Li's paper in 2020.

References

Li, X., Zhang, C., Xu, X., Miao, J., Yao, J., Liu, R., Zhao, Y., Chen, X., & Yang, Y. (2020). A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells. Nucleic acids research, 48(6), e33. https://doi.org/10.1093/nar/gkaa044