Composite
Part:BBa_K5276033
Designed by: Yuming Luo Group: iGEM24_UCAS-China (2024-10-01)
Selection plasmid
A118-rha_Promoter-PhiX174E-GFP
Fig1. Expression plasmid of traditional mutagenesis
Fig2. Selection plasmid of traditional mutagenesis
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 87
Illegal EcoRI site found at 843
Illegal EcoRI site found at 2380
Illegal EcoRI site found at 2695
Illegal XbaI site found at 83
Illegal XbaI site found at 2699
Illegal PstI site found at 849
Illegal PstI site found at 1002
Illegal PstI site found at 2221
Illegal PstI site found at 2374 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 87
Illegal EcoRI site found at 843
Illegal EcoRI site found at 2380
Illegal EcoRI site found at 2695
Illegal PstI site found at 849
Illegal PstI site found at 1002
Illegal PstI site found at 2221
Illegal PstI site found at 2374 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 87
Illegal EcoRI site found at 843
Illegal EcoRI site found at 2380
Illegal EcoRI site found at 2695
Illegal BamHI site found at 861
Illegal BamHI site found at 2362 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 87
Illegal EcoRI site found at 843
Illegal EcoRI site found at 2380
Illegal EcoRI site found at 2695
Illegal XbaI site found at 83
Illegal XbaI site found at 2699
Illegal PstI site found at 849
Illegal PstI site found at 1002
Illegal PstI site found at 2221
Illegal PstI site found at 2374 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 87
Illegal EcoRI site found at 843
Illegal EcoRI site found at 2380
Illegal EcoRI site found at 2695
Illegal XbaI site found at 83
Illegal XbaI site found at 2699
Illegal PstI site found at 849
Illegal PstI site found at 1002
Illegal PstI site found at 2221
Illegal PstI site found at 2374
Illegal AgeI site found at 138
Illegal AgeI site found at 261 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1376
Illegal BsaI.rc site found at 3048
[edit]
Categories
Parameters
None |