Composite

Part:BBa_K5276028

Designed by: Yuming Luo   Group: iGEM24_UCAS-China   (2024-10-01)


CRISPRi

J23119_dCas9_dbl term_Lac operon_sgRNA1_gRNA scaffold_T7 terminator_dbl term_Rha promoter_sgRNA2_gRNA scaffold_dbl term

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Figure 1. CRISPRi system
The CRISPRi system primarily consists of three promoters (the constitutive promoter J23111, the inducible lac operon promoter, and the Rha promoter) that respectively express dCas9 protein, sgRNA1, and sgRNA2. Additionally, it includes a gRNA scaffold, a T7 terminator, and a dbl terminator. By combining the CRISPRi system, the recombinase expression system (Tangram section), and the sequences above, we can achieve the transformation of logic gates by adding recombinant enzymes in different orders (Fig.2-3).
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Figure 2. Register A
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Figure 3. Register B

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1163
    Illegal NheI site found at 4382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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