Composite
Part:BBa_K5276028
Designed by: Yuming Luo Group: iGEM24_UCAS-China (2024-10-01)
CRISPRi
J23119_dCas9_dbl term_Lac operon_sgRNA1_gRNA scaffold_T7 terminator_dbl term_Rha promoter_sgRNA2_gRNA scaffold_dbl term
Figure 1. CRISPRi system
Figure 2. Register A
Figure 3. Register B
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1163
Illegal NheI site found at 4382 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3442
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |