Composite

Part:BBa_K5276026

Designed by: Yuming Luo   Group: iGEM24_UCAS-China   (2024-10-01)


Register A

Description
Register A is a unit of expression consisting of four promoters(constant promoter J23111, trc promoter, and inducible promoter lac operon, Rha promoter) with different directions, Three pairs of DNA recognition sites(TP901B-TC_TP901P-TC, Bxb1B-GT_Bxb1P-GT, TP901B-AG_TP901P-AG), a Ribosome binding site, and LuxI.
orthogonality-design-2.webp
Figure 1. TP901B-TC_trc promoter_lac promoter_TP901B-AG_Bxb1B-GT_TP901P-AG_J23111_Rha promoter_Bxb1P-GT_TP901P-TC_RBS_LuxI.
For Register A, the output will be A'+B if no inducers are added. Adding Ara (blue) results in A+B', and adding ATc (red) yields A'. Adding both inducers is also an option, but the order of addition is crucial. If Ara is added first followed by ATc, the output will be A+B. Conversely, if ATc is added before Ara, the output will be A.
orthogonality-design-2.webp
Figure 2. How Register A transforms into different logic gates.
Ara is the inducer of recombinase Tp901, while ATc is the inducer of recombinase bxbI. Different size of triangles represent different pairs of recombinase-recognition-sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 155
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 492
    Illegal NheI site found at 515
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 155
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 155
    Illegal NgoMIV site found at 335
    Illegal AgeI site found at 1120
    Illegal AgeI site found at 1243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355
    Illegal BsaI site found at 777


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Categories
Parameters
None