Part:BBa_K5276024

Tangram

P_BAD-TP901-P_LtetO-Bxb1-P_PhlF-A118-P_xylO-PhiC31

Figure 1: Tangram system

Tangram is an expression unit composed of four different inducible promoters(BAD promoter,LtetO promoter,Phlf promoter,xylO promoter) , each expressing a different recombinant enzyme(tp901,bxbI,A118,PhiC31).

Tangram is a dissection puzzle consisting of seven flat polygons which can be put together to form various shapes. We found this concept highly analogous to our design, where recombinases play the role of altering the shape of the gene circuits, thus enabling the execution of different logic gates.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 4068
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 318
  Illegal NheI site found at 341
  Illegal NheI site found at 1476
  Illegal SpeI site found at 4068
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 241
  Illegal BamHI site found at 1750
  Illegal XhoI site found at 1837
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 4068
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 4068
  Illegal AgeI site found at 76
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3471
  Illegal BsaI.rc site found at 2584
  Illegal SapI site found at 58
  Illegal SapI site found at 8196
  Illegal SapI.rc site found at 4464
  Illegal SapI.rc site found at 7887
  Illegal SapI.rc site found at 7945
  Illegal SapI.rc site found at 8050