Coding

Part:BBa_K5276010

Designed by: Zitao Qiu   Group: iGEM24_UCAS-China   (2024-10-01)


eMutaT7

MutaT7, initially a fusion of cytosine deaminase (AID) and T7 RNA polymerase (T7RNAP), enables mutations from dC→dT and deoxyguanosine (dG) to deoxyadenosine (dA) (caused by dC→dT mutations on the complementary strand). On this basis, when combined with adenosine deaminase, A→G and T→C mutations can be achieved (A:T→G:C). The enzyme, enhanced for mutagenic activity, is called eMutaT7(Park et al., 2021).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None