Part:BBa_K5276007

cymR

The cumate inducible promoter, consists of P_syn2 flanked by two CuO operator sequences; expression of a high-GC-content codon-optimized gene for the CymR repressor (cymR) is driven divergently by a modified *bla* promoter (P_bla-mut1T) in which the 35 box has been changed to TTGACA and the 10 box to TACAAT.

Nucleotide sequence of the P_Q5 promoter region is above. cymR and MCS start codons are in bold. Ribosome binding sites (RBS), operator sequences (CuO), and 35 and 10 promoter elements are boxed. Core promoter sequences of P_Q5 and the promoter driving cymR expression (P_bla-mut1T) are indicated by arrows. Translation of the MCS is indicated below the nucleotide sequence.

Reference

Kaczmarczyk, A., Vorholt, J. A., & Francez-Charlot, A. (2013). Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol, 79(21), 6795-6802.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 787
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 637
  Illegal SpeI site found at 787
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 787
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 787
  Illegal NgoMIV site found at 478
  Illegal NgoMIV site found at 511
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 626