Coding

Part:BBa_K5276004

Designed by: Zitao Qiu   Group: iGEM24_UCAS-China   (2024-10-01)


Gp44

Bacteriophages initially used recombination direction factors (RDF) to cut their own genes out of host DNA to form progeny phages. RDF can control the activity of recombinases to promote specific recombination reaction directions. Research indicates that the Gp44 protein(Mandali et al.,2017), as a recombination direction factor, can enhance the excision or inversion reaction of A118 between attL and attR sites.

For details about the specific principles of A118 recombinase and RDF, please refer to A118 recombinase (BBa_K5276001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 22
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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