Coding

Part:BBa_K5276003:Design

Designed by: Zitao Qiu   Group: iGEM24_UCAS-China   (2024-09-26)


PhiC31 recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1665
    Illegal SapI.rc site found at 1356
    Illegal SapI.rc site found at 1414
    Illegal SapI.rc site found at 1519


Design Notes

Source

References

Roquet, N., Soleimany, A. P., Ferris, A. C., Aaronson, S., & Lu, T. K. (2016). Synthetic recombinase-based state machines in living cells. Science, 353(6297), aad8559. doi:10.1126/science.aad8559

Grindley N. D. (1997). Site-specific recombination: synapsis and strand exchange revealed. Current biology : CB, 7(10), R608–R612. https://doi.org/10.1016/s0960-9822(06)00314-9