Protein_Domain

Part:BBa_K5271006

Designed by: Yin Yan Chan   Group: iGEM24_HKPOLYU   (2024-09-29)


Linker

A 3x GSSG linker connecting HER2 and EGFR nanobody domains.


Usage and Biology

The GS linker is a 12 amino acid long sequence containing 3x GSSG tandems connecting HER2 and EGFR nanobody. This basic part ensure a spatial separation between the two nanobody domains to achieve antigen binding or improve protein folding. The DNA sequence has performed codon optimization for expression in E.Coli.



Reference

  • Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357-1369.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/linker
Parameters
None