Signalling

Part:BBa_K5271004:Design

Designed by: Yin Yan Chan   Group: iGEM24_HKPOLYU   (2024-09-29)


Secretory peptide for Panobody


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The secretory sequence has mild to insignificant effect in facilitating nanobody with humanized Fc fragment. The nanobody containing this secretory peptide failed to folded into soluble form and secrete to the periplasm space.

Source

The DNA sequence of secretory peptide is obtained partially from the Heat-Stable Enterotoxin II (ssSTII) of E.Coli.

References

  • McCarthy, J. E., & Gualerzi, C. (1990). Translational control of prokaryotic gene expression. Trends in Genetics, 6, 78-85.
  • Simmons, L. C., & Yansura, D. G. (1996). Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli. Nature biotechnology, 14(5), 629-634.
  • Zhou, Y., Liu, P., Gan, Y., Sandoval, W., Katakam, A. K., Reichelt, M., ... & Reilly, D. (2016). Enhancing full-length antibody production by signal peptide engineering. Microbial Cell Factories, 15, 1-11.