Coding

Part:BBa_K527000

Designed by: Eva Meier Carlsen   Group: iGEM11_Copenhagen   (2011-09-17)

Biosynthesis/Oximes/CYP79A2

This BioBrick BBa_K527000 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.

CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of a cyanogenic glycoside. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 432


References

  • Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
  • Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.
[edit]
Categories
//cds/enzyme
Parameters
None