DNA

Part:BBa_K5269032

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


BsmB I insert

This part contains BsmB I cleavage sites and BsmB I recognition sequences at both ends for Golden Gate Assembly. The Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269025) is Golden Gate Assembly by this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None