Primer

Part:BBa_K5269031

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


shRNA-GGA_Site In-Fusion forward primer 2

This forward primer is used for T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(shRNA-GGA Site ,BBa_K5269025). This forwatd primer is used together with shRNA-GGA_Site In-Fusion reverse primer 2 (BBa_K5269030) for PCR and In-Fusion of shRNA-GGA Site to pBluescript II SK(-).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None