Primer

Part:BBa_K5269030

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


shRNA-GGA_Site In-Fusion reverse primer 2

This reverse primer is used for T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(shRNA-GGA Site ,BBa_K5269025). This reverse primer is used together with shRNA-GGA_Site In-Fusion forward primer 2 (BBa_K5269031) for PCR and In-Fusion of shRNA-GGA Site to pBluescript II SK(-).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
    Illegal SpeI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 26
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
    Illegal SpeI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
    Illegal SpeI site found at 11
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None