Part:BBa_K5269024
SpyTag-Glucanase-His-Tag and BsmB I cut site(for GGA)
It contains SD-sequences(BBa_K5269034), SpyTag, Endo-1,3(4)-b-glucanase(BBa_5269016) and His-Tag sequences. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-OmpA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, Endo-1,3(4)-b-glucanase, which hydrolyzes b-D-glucan contained in the target bacterial membrane, is expected to be stably present on the membrane vesicle, which will facilitate the introduction of shRNA into the target fungus cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 235
Illegal NotI site found at 1603 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2845
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 627
Illegal NgoMIV site found at 1252
Illegal NgoMIV site found at 1995
Illegal NgoMIV site found at 2620
Illegal AgeI site found at 385
Illegal AgeI site found at 1753 - 1000COMPATIBLE WITH RFC[1000]
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