Part:BBa_K5268040

Hyd-S12-1

Hyd-S12-1

Hyd-S12-1

Protein sequence:

MGGNLALKMAGEMGEGAPAALLGVCAVSPSVDLRETVRNLGRPPNRAYQWHFVRGLRRLVERKKGLYPRLYDTRRLDRLRTVRDFDELYTAPHGGFASADDYYSHSSALPLVAGIRVPTLIIHARDDPLVPCGPLLRPGATDNPYVAKATPAHGGHVAFVSADKGRRFWAEERLADFCRLPWGPPHA

Plasmid Profile:

PCR gel charts:

M: Marker, 2000 bp; Line17: Hyd-S12-1（564 bp）

Results of Western Blot:

M：Maker; C: CK; Line7: Hyd-S12-1, 20.62kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.648%

Line1: CK; Line3: Hyd-S12-1;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image:

Fourier Diagram:

Plastic Film(Carbonyl index(CI):2.32%)

Plastic Microspheres(Carbonyl index(CI):3.50%)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 170
  Illegal NgoMIV site found at 533
  
• 1000
  COMPATIBLE WITH RFC[1000]