DNA

Part:BBa_K5268008

Designed by: Yue Ke   Group: iGEM24_XJU-China   (2024-09-30)


Hyd-S11-1 belongs to the hydrolase enzyme family, and Hyd-S11-1 belongs to the polyethylene-degradin


Hyd-S11-1

Hyd-S11-1

Protein sequence:

MIRQLIEVDRVRTIAVAGYSLGGNLTLKLAGELADAAPPELKAVCAVSPTMDLAVCVEALERKSNILYEWNFVRNLKARMRLKSALWPGTFDLAGLRHVKTVRQFDDAYTAPHHGFRDAADYYHRASAMRIIDRIRIPALIVTAEDDPFVPAEPFRDPLVTNNPNLTVVVTPTGGHCAFVERAEPDYDGYWAEREIVRFATAHLAGPRAPQHPAKLMAL

Plasmid Profile:

Plasmid Profile

PCR Gel Charts:

M: Marker, 2000 bp; Line20: Hyd-S11-1(564 bp)

Results of Western Blot:

M: Maker; C: CK; Line6: Hyd-S11-1,24.36kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.423%

Line1: CK; Line6: Hdy-S11-1;

Scanning Electron Microscope Image:

Scanning Electron Microscope Image

Fourier Diagram:

Plastic Film(Carbonyl index(CI):2.22%)

Plastic Microspheres(Carbonyl index(CI):3.50%):

Plastic Microspheres(Carbonyl index(CI):3.50%)

Sequence and Features BBa_K5268002 SequenceAndFeatures

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 49
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None