Part:BBa_K5268005

Hyd-S11-1

Protein sequence:

MIRQLIEVDRVRTIAVAGYSLGGNLTLKLAGELADAAPPELKAVCAVSPTMDLAVCVEALERKSNILYEWNFVRNLKARMRLKSALWPGTFDLAGLRHVKTVRQFDDAYTAPHHGFRDAADYYHRASAMRIIDRIRIPALIVTAEDDPFVPAEPFRDPLVTNNPNLTVVVTPTGGHCAFVERAEPDYDGYWAEREIVRFATAHLAGPRAPQHPAKLMAL

Plasmid Profile:

PCR Gel Charts:

M: Marker, 2000 bp; Line20: Hyd-S11-1(564 bp)

Results of Western Blot:

M：Maker; C: CK; Line6: Hyd-S11-1,24.36kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.423%

Line1: CK; Line6: Hdy-S11-1;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image:

Fourier Diagram:

Plastic Film(Carbonyl index(CI):2.22%)

Plastic Microspheres(Carbonyl index(CI):3.50%)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 49
  
• 1000
  COMPATIBLE WITH RFC[1000]