DNA

Part:BBa_K5268001

Designed by: Yue Ke   Group: iGEM24_XJU-China   (2024-09-29)


Hyd-S11-1

Protein sequence: MIRQLIEVDRVRTIAVAGYSLGGNLTLKLAGELADAAPPELKAVCAVSPTMDLAVCVEALERKSNILYEWNFVRNLKARMRLKSALWPGTFDLAGLRHVKTVRQFDDAYTAPHHGFRDAADYYHRASAMRIIDRIRIPALIVTAEDDPFVPAEPFRDPLVTNNPNLTVVVTPTGGHCAFVERAEPDYDGYWAEREIVRFATAHLAGPRAPQHPAKLMAL Plasmid Profile:< img href="alkb-nj1-1-1.png"> PCR Gel Charts: < img href="alkb-nj1-1-2.png"> M: Marker, 2000 bp; Line20: Hyd-S11-1(564 bp) Results of Western Blot:< img href="alkb-nj1-1-3.png"> M:Maker; C: CK; Line6: Hyd-S11-1,24.36kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.423% < img href="alkb-nj1-1-4.png"> Line1: CK; Line6: Hdy-S11-1; Polyethylene film weight loss (0.01 < P < 0.05 labelled *, 0.001 < P <0.01 labelled **, P < 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns) Scanning Electron Microscope Image: < img href="alkb-nj1-1-5.png"> Fourier Diagram: < img href="alkb-nj1-1-6.png"> Plastic Film(Carbonyl index(CI):2.22%) < img href="alkb-nj1-1-7.png"> Plastic Microspheres(Carbonyl index(CI):3.50%)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 49
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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