Part:BBa_K5267011
mPGK Promoter
promoter that responsible for transcription of MTNR1a Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 116
- 1000COMPATIBLE WITH RFC[1000]
Profile
Name: mPGK promoter
Base Pairs: 516bp
Origin: Mouse
Properties: promoter that responsible for transcription of MTNR1a
Usage and Biology
MPGK promoter(mpPGK) is the promoter of Pgk1 in mouse cell. Pgk-1 is the murine gene encoding phosphoglycerate kinase (PGK), a key enzyme in glycolysis. This enzyme must be present in all cells, so the Pgk-1 promoter is expected to be a ubiquitously active one[1].
The cloned mpPGK is very active in driving transcription of reporter genes following transfection into mammalian cells[2]. The promoter is particularly active in pluripotent embryonal carcinoma and embryonic stem cells.
An initial studie on the Pgk-1 promoter indicated that its activity was contained within a region from −425 bp to +80 bp relative to the first transcription start site[2]. This promoter is GC rich and contains no TATA box[3]. The data from John P. Thomson et al. indicate that a primary function of non-methylated CGIs is to genetically influence the local chromatin modification state to initiate transcriptionby interaction with Cfp1 and perhaps other CpG-binding proteins[4]. The core promoter from −121 bp to +80 bp contains a number of putative Spl binding sites and had modest promoter activity. The 304 bp upstream of the core promoter had enhancer-like activity such that this region increased expression from the core promoter in an orientation- and position-independent fashion.
Figure
Top:
[1] SUTHERLAND L C, ST-ARNAUD R, MCBURNEY M W. An upstream activator sequence regulates the murine Pgk-1 promoter and binds multiple nuclear proteins [J]. Gene expression, 1995, 4(4-5): 265-79.
Regulated expression from the Pgk-1 promoter carrying the UAS. The Pgk-1 promoter comprising the region spanning −425 to +80 bp (black bars) and −121 to +80 bp were used to drive the lacZ reporter gene. (A) These constructs were cotransfected into P19 cells along with an RSV-driven CAT construct and harvested 48 h later. The P19 cells were treated with retinoic acid (RA) for the number of days shown before cells were harvested for measurement of β-galactosidase and CAT activities. (B) The two test constructs were cotransfected along with a selectible gene, Pgk-puro, and stably transformed cells were selected in puromycin. Colonies of transformed cells were pooled and treated with RA for the number of days shown before being harvested for β-galactosidase and CAT activities. The activity shown is that of β-galactosidase normalized to the CAT control.[1]
Sequence
GGAGCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGC
CTCTGGCCTCGCACACATTCCACATCCACCGGTAGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTA
GTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCAGTAGTCTCGTGCAGATGGACAG
CACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGG
GTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAG
CGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTCCAGCCCTACT
Reference
[2] MCBURNEY M W, SUTHERLAND L C, ADRA C N, et al. The mouse Pgk-1 gene promoter contains an upstream activator sequence [J]. Nucleic acids research, 1991, 19(20): 5755-61.
[3] ADRA C N, BOER P H, MCBURNEY M W. Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter [J]. Gene, 1987, 60(1): 65-74.
[4] THOMSON J P, SKENE P J, SELFRIDGE J, et al. CpG islands influence chromatin structure via the CpG-binding protein Cfp1 [J]. Nature, 2010, 464(7291): 1082-U162.
| None |