Composite

Part:BBa_K5257025:Design

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


NOT(TetR,TEV)-mRFP1magenta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 667
    Illegal AgeI site found at 779
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization of the linker for its synthesis was performed by replacing the sequence of ggaggaggaggtagt (BBa_K2549053) with ggaggaggtggatca (GGGGS). Also added a scar (tactag) between BBa_B0034 and BBa_K1162009



Source

Only iGEM parts, Each iGEM part was previously characterized by bibliography.


References