Composite

Part:BBa_K5257023:Design

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


LOT-MerA-GDH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
    Illegal NgoMIV site found at 2370
    Illegal AgeI site found at 879
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1265
    Illegal BsaI.rc site found at 3391


Design Notes

Codon optimization of the G4S linker (BBa_K2549053) was performed for synthesis in each module: LOT-DogCatcher (ggtggtggaggttct), DogTag-MerA_2D (ggaggaggtggatca) and gdh-DogTag (ggaggaggaggttca).



Source

Only iGEM parts, Each iGEM part was previously characterized by bibliography.


References