Composite

Part:BBa_K5257023

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


LOT-MerA-GDH

A system that visualizes the MerA enzyme on the cell surface without the addition of the cofactor NADPH.

The LOT anchor motif is post-translationally fused to the MerA enzyme (BBa_K5257022) and the GDH enzyme (BBa_K5257020) by the DogTag/DogCatcher system. The MerA enzyme will volatilize inorganic mercury (Hg2+) before it enters the cell, the enzyme requires the cofactor NADPH which will be provided by the GDH enzyme, thus no external addition of the cofactor will be necessary, which is a great advantage.

The iGEM Bolivia team will use it for a novel organic mercury biosensor. However, this part can also be used in bioremediation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 718
    Illegal PstI site found at 1330
    Illegal NgoMIV site found at 2370
    Illegal AgeI site found at 879
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1265
    Illegal BsaI.rc site found at 3391


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Categories
Parameters
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