Coding

Part:BBa_K5257022

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


DogTag-MerA_2D

The MerA_2D (BBa_K5257007) enzyme is expected to localize on the cell surface to volatilize inorganic mercury (Hg2+) and prevent it from entering the cell, the enzyme requires the cofactor NADPH which can be provided externally or through the enzyme gdh-DogTag (BBa_K5257020).

This part includes a degradation system controlled by the TEV enzyme (BBa_K5257001).

The iGEM Bolivia team will use it for a novel organic mercury biosensor. However, this part can also be used in bioremediation.

Modular system inspired by Gallus et al. (2020).

Reference

Gallus, S., Peschke, T., Paulsen, M., Burgahn, T., Niemeyer, C. M., & Rabe, K. S. (2020). Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. ChemBioChem, 21(15), 2126-2131. https://doi.org/10.1002/cbic.202000102


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 341
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 341
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 341
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 341
    Illegal NgoMIV site found at 1381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 276


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Categories
Parameters
None