Coding

Part:BBa_K5257020

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


gdh-DogTag

gdh-DogTag is fused to LOT-DogCatcher (BBa_K5257019). The gdh enzyme is expected to localize to the cell surface to provide the cofactor NADPH to other enzymes.

Modular system inspired by Gallus et al. (2020).

Reference

Gallus, S., Peschke, T., Paulsen, M., Burgahn, T., Niemeyer, C. M., & Rabe, K. S. (2020). Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. ChemBioChem, 21(15), 2126-2131. https://doi.org/10.1002/cbic.202000102

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 508


[edit]
Categories
Parameters
None