Coding
Part:BBa_K5257020
Designed by: Oskar Samuel Zeballos Huarachi Group: iGEM24_Bolivia (2024-09-29)
gdh-DogTag
gdh-DogTag is fused to LOT-DogCatcher (BBa_K5257019). The gdh enzyme is expected to localize to the cell surface to provide the cofactor NADPH to other enzymes.
Modular system inspired by Gallus et al. (2020).
Reference
Gallus, S., Peschke, T., Paulsen, M., Burgahn, T., Niemeyer, C. M., & Rabe, K. S. (2020). Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. ChemBioChem, 21(15), 2126-2131. https://doi.org/10.1002/cbic.202000102
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 508
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Categories
Parameters
None |