Coding

Part:BBa_K5257019

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


LOT-DogCatcher

Cell surface protein visualization module using the DogTag/DogCatcher system (BBa_K5257012/BBa_K5257013) and the LOT anchor motif (BBa_K5257009).

Modular system inspired by Gallus et al. (2020).

Reference

Gallus, S., Peschke, T., Paulsen, M., Burgahn, T., Niemeyer, C. M., & Rabe, K. S. (2020). Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. ChemBioChem, 21(15), 2126-2131. https://doi.org/10.1002/cbic.202000102


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 637
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 637
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 637
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 637
    Illegal AgeI site found at 798
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None