Tag

Part:BBa_K5257013:Design

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


DogCatcher


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 73
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 73
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 73
    Illegal AgeI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Keeble, A. H., Yadav, V. K., Ferla, M. P., Bauer, C. C., Chuntharpursat-Bon, E., Huang, J., & Howarth, M. (2022). DogCatcher allows loop-friendly protein-protein ligation. Cell chemical biology, 29(2), 339-350. https://doi.org/10.1016/j.chembiol.2021.07.005


References